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. 2019 Feb 27;8(5):e1581546. doi: 10.1080/2162402X.2019.1581546

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Double staining immunofluorescence of VISTA (red signals) and CD68, CD4 and CD8 (green signals) with counterstaining of the nuclei with DAPI (blue signals). The photos (a-c) represent one double staining each with different fluorescence filters. (a) VISTA and CD68 show a coexpression representing myeloid origin; (b) VISTA and CD4 show a strong coexpression within the VISTA positive TILs; (c) No correlation of VISTA and CD8 was seen.