Figure 2.

Effect of BKM120 treatment on the growth of VMCUB1, PIK3CA-mutated and HT1376, PIK3CA-wild type human bladder tumors in humanized mice.

a. Experimental protocol: NSG mice were s.c. grafted with the indicated tumor cell line, and when tumors were palpable, they were i.v. injected with PBMCs. Three days later, mice were randomized to the “untreated” (N = 14) or “BKM120-treated” (N = 14) groups. BKM120 was administered daily at a dose of 30mg/kg by oral gavage.b-c. Growth kinetics (b: individual curves; c: mean curves + standard error of the mean) of VMCUB1 (left panel) and HT1376 (right panel) tumors; untreated (black lines), BKM120-treated (blue lines). Tumors were measured twice per week.d. GvHD was followed by the loss of body weight, shown as a percentage of initial weight (mean curves). Results shown are the pool of two independent experiments with similar results. Statistical significance was calculated using the Mann–Whitney test. NS, not significant; ***, P < 0.0005.