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. 2019 Mar 16;8(5):e1581556. doi: 10.1080/2162402X.2019.1581556

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — Effect of BKM120 treatment on the tumor immune infiltrate of VMCUB1, PIK3CA-mutated, and HT1376, PIK3CA-wild type human bladder tumors in humanized mice.

Humanized mice were treated as in Figure 2 and tumors were analyzed at day 25.a. FACS analysis showing the proportion of total HuCD45+ and CD3+ T cells infiltrating the tumor, among live cells. Left panels: PIK3CA-mutated VMCUB1 tumors (N = 8 in each group); right panels: PIK3CA-wild type HT1376 tumors (N = 6 in the untreated group and N = 7 in the BKM120 group).b. Proportion of the indicated immune cell subsets among tumor-infiltrating HuCD45+ cells. Results shown are from one representative experiment out of two with similar results. Statistical significance was calculated using the Mann–Whitney test. NS, not significant; *, P < 0.05; **, P < 0.01.C. VMCUB1 PIK3CA-mutated tumors from untreated and BKM120-treated mice were characterized by IHC for human infiltrate (HuCD45). Representative IHC stainings of two mice per indicated group are shown with two different magnifications: smaller square, x50; larger square, x400.