Figure 4.

Immune gene expression changes induced by BKM120 treatment on PIK3CA-mutated VMCUB1 and PIK3CA-wild type HT1376 human bladder tumors in humanized mice.

NSG humanized mice were treated with BKM120 or untreated, as detailed in Figure 2 and tumors were analyzed at day 25. Shown is the mRNA expression quantified by qRT-PCR of the indicated genes in PIK3CA-mutated VMCUB1 tumors (left panels) and PIK3CA-wild type HT1376 tumors (right panels), untreated (black dots) or BKM120-treated (blue dots). PIK3CA-mutated VMCUB1 tumors: N = 13 in the untreated group and N = 15 in the BKM120 group. PIK3CA-wild type HT1376 tumors: N = 12 in the untreated group and N = 13 in the BKM120 group. Statistical significance was calculated using the Mann–Whitney test; * p < 0.05, ** p < 0.01.