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. 2014 Mar 15;20(6):521–527. doi: 10.1111/cns.12248

Figure 1.

Figure 1

Calpain small subunit 1 (Capn4) expression is increased in gliomas and positively correlated with histological malignancy. Tissue microarrays (TMAs) and formalin‐fixed and paraffin‐embedded (FFPE) tissue containing control brain (n = 21) and glioma specimens (n = 279) were subjected to immunohistochemical staining with the Capn4 antibody as described in the Methods section. (A) Hematoxylin‐eosin and immunohistochemistry. Weak Capn4 staining was observed in control brain tissues. Capn4 protein expression was higher in tumor samples. Scale bar: 100 μm and 25 μm. (B) Capn4 expression was significantly higher in gliomas than in control brain tissues. (C) Capn4 expression varied with glioma grade. (D) The level of Capn4 was 8.70% (2/23) in grade I, 44.23% (46/104) in grade II, 62.96% (17/27) in grade III, and 56.00% (70/125) in grade IV. (E) Capn4 expression was positively correlated with glioma grade.*P < 0.05; **P < 0.01; ***P < 0.001. N.S. indicates no significance.