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. 2013 Aug 24;20(1):59–66. doi: 10.1111/cns.12161

Figure 1.

Figure 1

AChE induces Aβ aggregation and deposition. (A) ThT assay of AChE inducing Aβ aggregation. Solution of 125 μM Aβ in the presence and absence of 1.25 μM AChE were incubated in 0.215 M PB (PH 8.0) at 37°C for 24 h. Solutions were diluted with 10 μM ThT and the fluorescence was monitored on Varioskan Flash at an excitation of 446 nm and emission of 490 nm. Data are the mean values ± SEM (n = 4). ***P < 0.001, # # # P < 0.001. (B) Effects of AChE on inducing Aβ deposition by TEM experiments. Aβ was incubated alone or co‐incubated with AChE for 7 days at 37°C; AChE was incubated alone as a control. Samples were dropped into the grids, stained with 2% filtered uranyl acetate. Arrows indicate Aβ fibrils, senile plaques and G4 AChE, respectively. The morphologies were imaged by JOEL JEM‐1230 TEM at 80 kV. The scale bars represent 100 nm.