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. 2013 Aug 24;20(1):59–66. doi: 10.1111/cns.12161

Figure 2.

Figure 2

Identification of the oligomeric structure in AChE by dot‐blot assay. 230 μM Aβ was incubated alone or with 2.3 μM AChE in 0.215 M PB (pH 8.0) for (A) 1 h, 48 h, 72 h and 120 h at 37°C. (B) AChE was incubated in the presence or absence of 230 μM Aβ for 48 h at 37°C. Samples were applied to a nitrocellulose membrane and probed with either oligomer‐specific antibody A11 or sequence‐specific antibody 6E10. Buffer was used as control.