Skip to main content
letter
. 2013 Dec 3;20(1):95–97. doi: 10.1111/cns.12211

Figure 1.

Figure 1

Voltage‐dependent potassium currents composed of delayed rectifier potassium current (I K) and transient outward potassium current (I to), and effects of humanin and anoxia on I‐V curves of I K and I to. Neurons were held at a holding potential of −70 mV, and potassium currents (A) were elicited by a series depolarizing pulses as follows: Hyperpolarizing prepulse to −110 mV was followed by depolarizing test pulses with a frequency of 0.1 Hz from −70 to 40 mV in 10 mV increment (M). To get I K (B), 4‐aminopyridine 1 mmol/L was added into external solution to abolish I to. I to (C) was isolated by subtracting current trace of I K from potassium currents utilizing the signal subtraction procedure in Pulse 8.5 software. Being normalized with respect to maximal value, currents amplitudes were expressed as mean ± SD and were analyzed by one‐way ANOVA Dunnett's test. *P < 0.05,versus controls. n = 10 neurons in each group. Amplitudes of I K (D) were decreased by humanin and humanin+anoxia significantly in membrane potentials of 20~40 mV, while amplitudes of I to (E) were decreased by anoxia significantly in membrane potentials of 0~40 mV.