Table 1.
EVs proteins recovered from UC samples and ODG fractions (F1 to F8) compared with top 100 proteins from ExoCarta website. Proteins were ranked according to their abundancy of identification in exocarta. Protein IDs and gene names are reported. In bold: proteins found at least one time in EVs issued from leech microglia. Underlined in red: proteins from ODG EV-rich fractions, which are reported in Exocarta as microglial proteins.
| ODG Fractions |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Protein IDs | Gene name | Protein name | UC | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 |
| CD9 | CD9 | - | - | - | - | - | - | - | - | - | |
| PDCD6IP | Programmed cell death 6-interacting protein | - | - | - | - | - | - | - | - | - | |
| P11142 | HSPA8 | Heat shock cognate 71 kDa protein | - | + | + | + | + | + | + | + | + |
| P04406 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | + | + | + | + | + | + | + | + | + |
| P60709 | ACTB | Actin, cytoplasmic 1 | + | + | + | + | + | + | + | + | + |
| P07355 | ANXA2 | Annexin A2 | - | + | + | + | + | + | + | + | + |
| CD63 | CD63 antigen | - | - | - | - | - | - | - | - | - | |
| SDCBP | Syntenin-2 | - | - | - | - | - | - | - | - | - | |
| P06733 | ENO1 | Alpha-enolase | + | + | + | + | + | + | + | + | - |
| P07900 | HSP90AA1 | Heat shock protein HSP 90-alpha | + | + | + | - | + | + | + | + | - |
| TSG101 | Tumor susceptibility gene 101 protein | - | - | - | - | - | - | - | - | - | |
| P14618 | PKM | Pyruvate kinase PKM | + | - | + | + | + | + | + | + | - |
| P00338 | LDHA | L-lactate dehydrogenase A chain, LDH-A | + | - | - | - | + | + | + | - | - |
| Q5VTE0 | EEF1A1 | Elongation factor 1-alpha 1 | - | + | + | + | + | + | + | + | + |
| P63104 | YWHAZ | 14-3-3 protein zeta/delta | - | - | - | - | + | - | + | - | - |
| P00558 | PGK1 | Phosphoglycerate kinase 1 | - | - | - | - | + | + | - | - | - |
| P13639 | EEF2 | Elongation factor 2 | - | - | + | + | + | + | + | + | - |
| P04075 | ALDOA | Fructose-bisphosphate aldolase A | + | - | + | + | + | + | + | + | + |
| P08238 | HSP90AB1 | Heat shock protein HSP 90-beta | + | - | + | + | + | + | + | + | - |
| P08758 | ANXA5 | Annexin A5 | + | - | + | + | + | + | + | + | + |
| P49327 | FASN | Fatty acid synthase | - | - | - | - | - | - | + | - | - |
| P62258 | YWHAE | 14-3-3 protein epsilon | + | - | - | - | + | + | + | - | - |
| Q00610 | CLTC | Clathrin heavy chain 1 | + | - | - | - | + | + | + | + | - |
| CD81 | CD81 antigen | - | - | - | - | - | - | - | - | - | |
| ALB | Serum albumin | - | - | - | - | - | - | - | - | - | |
| P55072 | VCP | Transitional endoplasmic reticulum ATPase | + | - | - | - | + | + | + | - | - |
| P60174 | TPI1 | Triosephosphate isomerase | + | - | - | - | + | + | + | + | - |
| P62937 | PPIA | Peptidyl-prolyl cis-trans isomerase A | + | - | - | + | + | + | + | - | - |
| P26038 | MSN | Moesin | + | - | - | - | + | + | - | - | - |
| CFL1 | Cofilin-1 | - | - | - | - | - | - | - | - | - | |
| Q06830 | PRDX1 | Peroxiredoxin-1 | + | - | + | + | + | + | + | + | + |
| PFN1 | Profilin-1 | - | - | - | - | - | - | - | - | - | |
| P61224 | RAP1B | Ras-related protein Rap-1b | + | - | - | + | + | + | + | + | - |
| ITGB1 | Integrin beta-1 | - | - | - | - | - | - | - | - | - | |
| P11021 | HSPA5 | Endoplasmic reticulum chaperone BiP | + | - | - | - | + | + | + | + | + |
| SLC3A2 | 4F2 cell-surface antigen heavy chain, 4F2hc | - | - | - | - | - | - | - | - | - | |
| P62805 | HIST1H4A | Histone H4 | - | + | + | + | + | + | + | + | + |
| GNB2 | Guanine nucleotide-binding protein | - | - | - | - | - | - | - | - | - | |
| P05023 | ATP1A1 | Sodium/potassium-transporting ATPase subunit alpha-1 | + | - | + | + | + | + | + | + | + |
| P27348 | YWHAQ | 14-3-3 protein theta | + | - | + | + | + | + | + | + | - |
| O75955 | FLOT1 | Flotillin-1 | + | - | - | - | + | + | + | - | - |
| P21333 | FLNA | Filamin-A | + | - | - | - | + | + | + | - | - |
| O00299 | CLIC1 | Chloride intracellular channel protein 1 | - | - | - | - | + | - | + | - | - |
| P78371 | CCT2 | T-complex protein 1 subunit beta | - | - | - | - | + | + | - | - | - |
| P60953 | CDC42 | CDC42 small effector protein 1 | + | - | - | - | + | + | + | - | - |
| P61981 | YWHAG | 14-3-3 protein gamma | - | - | - | - | - | - | + | - | - |
| P01023 | A2M | Alpha-2-macroglobulin, Alpha-2-M | - | + | - | - | - | - | - | - | - |
| TUBA1B | Tubulin alpha-1B chain | - | - | - | - | - | - | - | - | - | |
| RAC1 | Ras-related C3 botulinum toxin substrate 1 | - | - | - | - | - | - | - | - | - | |
| LGALS3BP | Galectin-3-binding protein | - | - | - | - | - | - | - | - | - | |
| P0DMV9 | HSPA1A | Heat shock 70 kDa protein 1A | - | - | - | - | - | - | + | - | - |
| GNAI2 | Guanine nucleotide-binding protein G(i) subunit alpha-2 | - | - | - | - | - | - | - | - | - | |
| P04083 | ANXA1 | Annexin A1 | - | - | - | + | - | - | + | - | - |
| P61586 | RHOA | Transforming protein RhoA | - | - | - | - | + | + | + | - | - |
| MFGE8 | Lactadherin | - | - | - | - | - | - | - | - | - | |
| P32119 | PRDX2 | Peroxiredoxin-2 | + | - | - | - | + | + | + | + | - |
| P50395 | GDI2 | Rab GDP dissociation inhibitor beta | - | - | - | + | + | + | + | + | - |
| EHD4 | EH domain-containing protein 4 | - | - | - | - | - | - | - | - | - | |
| O43707 | ACTN4 | Alpha-actinin-4 | - | - | - | - | + | - | + | - | - |
| P31946 | YWHAB | 14-3-3 protein beta/alpha | - | - | - | - | - | - | + | - | - |
| P51149 | RAB7A | Ras-related protein Rab-7a | + | - | - | - | + | - | + | - | - |
| LDHB | L-lactate dehydrogenase B chain | - | - | - | - | - | - | - | - | - | |
| P63092 | GNAS | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | - | - | - | - | + | + | - | - | - |
| RAB5C | Ras-related protein Rab-5C | - | - | - | - | - | - | - | - | - | |
| ARF1 | ADP-ribosylation factor GTPase-activating protein 1 | - | - | - | - | - | - | - | - | - | |
| ANXA6 | Annexin A6 | - | - | - | - | - | - | - | - | - | |
| P50995 | ANXA11 | Annexin A11 | + | - | - | + | + | + | + | + | - |
| P63261 | ACTG1 | Actin, cytoplasmic 2 | - | + | + | + | + | + | + | + | + |
| KPNB1 | Importin subunit beta-1 | - | - | - | - | - | - | - | - | - | |
| EZR | Ezrin | - | - | - | - | - | - | - | - | - | |
| P09525 | ANXA4 | Annexin A4 | + | - | - | - | + | + | + | - | - |
| P53396 | ACLY | ATP-citrate synthase | - | - | - | - | + | - | - | - | - |
| Q9BQE3 | TUBA1C | Tubulin alpha-1C chain | - | - | - | - | - | - | + | - | - |
| P02786 | TFRC | Transferrin receptor protein 1 | - | - | - | - | + | + | + | - | - |
| RAB14 | Ras-related protein Rab-14 | - | - | - | - | - | - | - | - | - | |
| P62805 | HIST2H4A | Histone H4 | - | + | + | + | + | + | + | + | + |
| GNB1 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | - | - | - | - | - | - | - | - | - | |
| THBS1 | Thrombospondin-1 | - | - | - | - | - | - | - | - | - | |
| RAN | GTP-binding nuclear protein Ran | - | - | - | - | - | - | - | - | - | |
| RAB5A | Ras-related protein Rab-5A | - | - | - | - | - | - | - | - | - | |
| PTGFRN | Prostaglandin F2 receptor negative regulator | - | - | - | - | - | - | - | - | - | |
| P48643 | CCT5 | T-complex protein 1 subunit epsilon | - | - | - | - | - | - | - | + | - |
| P49368 | CCT3 | T-complex protein 1 subunit gamma | - | - | - | - | + | + | + | - | - |
| P23526 | AHCY | Adenosylhomocysteinase | + | - | - | - | + | + | + | - | - |
| P22314 | UBA1 | Ubiquitin-like modifier-activating enzyme 1 | - | - | - | - | + | + | + | - | - |
| RAB5B | Ras-related protein Rab-5B | - | - | - | - | - | - | - | - | - | |
| RAB1A | Ras-related protein Rab-1A | - | - | - | - | - | - | - | - | - | |
| LAMP2 | Lysosome-associated membrane glycoprotein 2 | - | - | - | - | - | - | - | - | - | |
| P23229 | ITGA6 | Integrin alpha-6 | - | - | - | + | + | + | + | - | - |
| P62805 | HIST1H4B | Histone H4 | - | + | + | + | + | + | + | + | + |
| P35613 | BSG | Basigin | - | - | - | - | - | + | - | - | - |
| YWHAH | 14-3-3 protein eta | - | - | - | - | - | - | - | - | - | |
| Q71U36 | TUBA1A | Tubulin alpha-1A chain | - | - | - | - | - | - | + | + | - |
| P29401 | TKT | Transketolase | - | - | - | - | - | + | + | - | - |
| TCP1 | T-complex protein 1 | - | - | - | - | - | - | - | - | - | |
| STOM | Erythrocyte band 7 integral membrane protein | - | - | - | - | - | - | - | - | - | |
| SLC16A1 | Monocarboxylate transporter 1 | - | - | - | - | - | - | - | - | - | |
| RAB8A | Ras-related protein Rab-8A | - | - | - | - | - | - | - | - | - | |
| P35579 | MYH9 | Myosin-9 | - | - | - | - | - | - | + | - | - |
| Q14764 | MVP | Major vault protein | + | - | - | - | - | + | + | + | - |
| Total | 100 | 29 | 12 | 19 | 24 | 50 | 48 | 56 | 29 | 14 | |
| 64 | |||||||||||