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Recovery and matrix effect of the method for 1 D and IS in rat plasma (n = 5).
| Compd. | Spiked concentration (ng/mL) | Recovery |
Matrix effect |
||
|---|---|---|---|---|---|
| mean ± SD (%) | RSD (%) | mean ± SD (%) | RSD (%) | ||
| 1D | 5 | 103.7 ± 6.0 | 5.8 | 97.3 ± 6.3 | 6.5 |
| 10 | 105.9 ± 10.6 | 10.0 | 97.5 ± 9.7 | 9.9 | |
| 100 | 102.5 ± 6.9 | 6.8 | 91.3 ± 0.8 | 0.9 | |
| 500 | 103.5 ± 7.7 | 7.4 | 95.3 ± 2.6 | 2.8 | |
| IS | 400 | 108.4 ± 4.9 | 4.5 | 91.0 ± 2.7 | 3.0 |
