Table 3.

Stability of the method for 1 D in rat plasma (n = 5).

Spiked concentration (ng/mL)	Condition	Measured concentration (mean ± SD, ng/mL)	RSD (%)	Accuracy (%)
10	Control	10.34 ± 0.7	6.8	103.4
	6 h at 25 °C	10.27 ± 0.56	5.5	102.7
	4 weeks at −20 °C	10.61 ± 0.55	5.2	106.1
	Three freeze-thaw cycles	10.12 ± 0.66	6.5	101.2
	Autosampler for 24 h	10.36 ± 0.75	7.2	103.6
100	Control	100.27 ± 7.02	7.0	100.3
	6 h at 25 °C	104.71 ± 5.16	4.9	104.7
	4 weeks at −20 °C	98.39 ± 7.52	7.6	98.4
	Three freeze-thaw cycles	99.36 ± 6.92	7.0	99.4
	Autosampler for 24 h	101.51 ± 6.43	6.3	101.5
500	Control	520.75 ± 35.48	6.8	104.2
	6 h at 25 °C	530.28 ± 34.32	6.5	106.1
	4 weeks at −20 °C	518.57 ± 43.30	8.4	103.7
	Three freeze-thaw cycles	503.88 ± 33.79	6.7	100.8
	Autosampler for 24 h	481.19 ± 42.98	8.9	96.2