Skip to main content
. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Glia. 2018 Dec 24;67(5):802–824. doi: 10.1002/glia.23568

Figure 7. Inflammasome activation by HIV ssRNA40 induces autophagy receptor expression in microglial cells.

Figure 7

(a) Expression analysis of autophagy receptors by qPCR following 24h exposure of HMG cells to ssRNA40. Results are presented as mean ± SD, n=3; *P<0.05. (b) Top, representative immunoblots showing expression of different autophagy receptors in HMG cells at 24h post treatment with ssRNA40. Bottom, densitometric analysis for 3 independent donors showing fold changes in expression of SQSTM1, NDP52 and OPTN in ssRNA40 stimulated cells compared to vehicle treated cells. (c) Top, representative immunoblots for PINK1, Parkin and β-Actin expression in lysates from HMG stimulated with ssRNA40, ssRNA41 or vehicle for 24h. Bottom, densitometric analysis for three independent donors showing fold change in expression of PINK1 and Parkin in ssRNA40 treated cells compared to vehicle treated cells. Results are presented as mean ± SD, n=3; *P<0.05.

HHS Vulnerability Disclosure