Skip to main content
. Author manuscript; available in PMC: 2019 Oct 11.
Published in final edited form as: Proteomics. 2018 Oct 11;18(20):e1800190. doi: 10.1002/pmic.201800190

Figure 4. Cell proliferation was suppressed in response to caffeine treatment.

Figure 4.

(A-B) TRT-HU1, hTert-immortalized normal bladder epithelial cells, were treated with 0.005, 0.05, and 0.1 mM caffeine for 24, 48, or 72 hrs. (A) Cell counting and (B) crystal violet proliferation assay were conducted as described in Methods. *P<0.05 (two-sided Student’s t-test) compared with the control group. Representative images of TRT-HU1 cells treated with caffeine (lower panels). (C) Seahorse data showed that caffeine treatment did not alter metabolism of normal bladder epithelial cells. Top, oxygen consumption rate (OCR) chart *p < 0.05. Bottom, extracellular acidification rate (ECAR) chart ** p < 0.05. (D) Quantification results from western blot analysis to measure the expression levels of ACTG2, HIST1H2BM, ACTA2, MYH2, MYH7B and histone H2B proteins in the presence or absence of caffeine. β-actin was used loading control. The differentially expressed protein levels obtained from proteomics analysis were shown in Table (right).