Figure 1.
Experimental design. Mice were subjected to two separate study groups (A, black and white squares). Day 0 refers to surgery day. Mice were trained on the accelerated rotarod daily from day −5 to day 0 to generate stable baseline values. Baseline values before surgery were obtained also for neuroscore, negative geotaxis, and beam walk on day −1. All the other tests were performed after surgery. tMCAo mice showed a significant loss of body weight (B) compared with sham‐operated mice at all time points considered. Body weight in the two independent groups of mice (represented with white and black boxes) was never different confirming a comparable degree of injury. Two‐way ANOVA with RM followed by post hoc Tukey's test: **P < 0.01, ***P < 0.001 tMCAo compared with sham‐operated mice.