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. 2013 Mar 25;19(4):235–243. doi: 10.1111/cns.12069

Figure 3.

Figure 3

Cell viability and inhibition of nitrogen monoxide (NO), tumor necrosis factor α (TNF‐α), and IL‐1β production in lipopolysaccharide (LPS)‐induced microglial cells. The cells were incubated with different concentration of resolvin D1 (RvD1) and treated with LPS in the absence or presence of Boc‐2. (A) MTT method was used to analyze the cell viability. The results showed that cell viability was reduced by 15% after cells were incubated in LPS (100 ng/mL) for 24 h, and with no effect on the pretreatment of RvD1 (100, 10, and 1 nM). (B, C and D) Bio‐Rad absorbance reader and ELISA were used to analyze the production of NO, TNF‐α, and IL‐1β, and these production significantly inhibited by 10 and 100 nM RvD1, with the inhibition of Boc‐2 in the process of antiinflammation. **P < 0.01 compared with control cells; ## P < 0.01 compared with LPS in the absence of RvD1.