Figure 3.

The effects of intracerebroventricular administration of the CB1 receptor agonist, ACEA, and leptin on the mean spike frequency of penicillin‐induced epileptiform activity and their interaction. (A) ACEA, at a dose of 7.5 μg, significantly decreased the mean frequency of epileptiform activity in the 50 min after ACEA injection. The administration of leptin (1 μg) 10 min before ACEA (7.5 μg) inhibited the anticonvulsant effect of ACEA. The proconvulsant activity appeared within 80 min after leptin injection. (B) ACEA, at a dose of 2.5 μg, did not influence the mean frequency of epileptiform activity. The administration of leptin (1 μg) 10 min before ACEA (2.5 μg) caused proconvulsant effect on the mean spike frequency of epileptiform activity within 80 min after leptin injection. *P < 0.05, **P < 0.01, ***P < 0.001. The percentage frequency of epileptiform ECoG activity value depends on both the frequency of epileptiform ECoG activity before and after the substance administered as it is defined as follows:

$$\mathrm{Frequency}\mathrm{Value}\mathrm{\%}=\frac{\mathrm{The}\mathrm{mean}\mathrm{of}\mathrm{spike}\mathrm{frequency}\mathrm{after}\mathrm{substance}\mathrm{administered}}{\mathrm{The}\mathrm{mean}\mathrm{of}\mathrm{spike}\mathrm{frequency}\mathrm{before}\mathrm{substance}\mathrm{administered}}\times 100$$