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. 2013 Feb 26;19(3):183–190. doi: 10.1111/cns.12059

Figure 3.

Figure 3

Pretreatment with 5‐aza‐dC increased the neurotoxic damages of MPP +, 6‐OHDA, and rotenone on dopaminergic neuronal cells. N27 cells were pretreated with 5‐aza‐dC (0.5 μM) for 24 h, followed by 12 h (A) or 24 h (B) of MPP + (200 μM), 6‐OHDA (15 μM) or rotenone (0.5 μM), then counted by trypan blue staining (upper panel) and visualized by morphological observation (lower panel). SH‐SY5Y cells were pretreated with 5‐aza‐dC (10 or 50 μM) for 24 h, followed by MPP + (400 μM) for 12 h (C) or for 24 h (D). N27 cells (E) were pretreated with 5‐aza‐dC (0.5 μM) for 24 h, followed by 12 h of MPP + (200 μM). Cell viability was assayed using a cell viability analyzer. The values represent the means ± SEM of three experiments (n = 3). *P < 0.05. 5aza, 5‐aza‐2′‐deoxycytidine; MPP, 1‐methyl‐4‐phenylpyridinium (MPP +); 6‐OHDA, 6‐hydroxydopamine.