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. 2013 Oct 25;19(12):926–936. doi: 10.1111/cns.12163

Figure 3.

Figure 3

Curcumin treatment decreased GLI1 expression at the cytoplasma,nucleus and inactivated consensus GLI1‐binding sites. (A) T98G cells treated with 20 uM of curcumin for 24 h were subjected to immunofluorescent staining using anti‐GLI1 antibody. Curcumin treatment prevented most of GLI1 translocation into nucleus and resulted in lower expression levels at the cytoplasma. Bars represent 20 um. (B) U87 and T98G cells were transiently transfected (24 h) with a firefly luciferase reporter construct containing eight consecutive consensus GLI1‐binding sites (8×‐GLI1) and cotransfected with pGL4.75. Cells were then treated as indicated with solvent DMSO (control) or curcumin. Both firefly and Renilla luciferase activities were quantified, and the firefly/Renilla luciferase activities were recorded as fold induction over solvent‐treated (Con) cells transfected with the 8×‐GLI1/pGL4.75 vector reporter constructs. Mean ± SEM (n = 3; *P < 0.05).