Figure 2.

Effects of risperidone in neuronal culture. (A) Effect of risperidone on cultured neurons exposed to H₂O₂: results from flow cytometry (n = 4 in each group). The histogram shows the percentage of apoptotic cells in the treatment groups versus the control group. Flow cytometry analysis of cell apoptosis (Annexin V staining, R2 + R3) and necrosis (PI staining, R3 + R4), R1, alive cells; R2, early apoptotic cells (Annexin V–positive, PI‐negative); R3, late apoptotic or necrotic cells (Annexin V–positive, PI‐positive); R4, dead cells and cell fraction (Annexin V–negative, PI‐positive). (B) Effect of risperidone on neurons exposed to OGD (upper) and H₂O₂ (lower): results from MTT assay (n = 6 in each group). The values of MTT absorbance and apoptosis rates are expressed as mean ± SD. Dunnett t‐test, *P < 0.05, **P < 0.01 for treatment group versus control group.