Fig 5. Bacteria colonization from mouse ileum samples after challenge with ETEC strain H10407.

Mice were challenged with H10407 after SC immunized with PBS (control), heat-extracted CFA/I fimbria (CFA/I), recombinant major subunit CfaB (CfaB), or recombinant tip subunit CfaE (CfaE), orally inoculated with H10407 (primed w/H10407), or orally primed with H10407 followed with SC boosters with CFA/I fimbriae (H10407 + CFA/I). Numbers of H10407 bacteria collected from mouse ileum samples were cultured and counted (CFUs in log₁₀). Data were normalized by log transformation and analyzed using One-way ANOVA with Tukey’s post hoc test. The line in each group represents the mean with standard deviation.