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. 2019 Apr 19;15(4):e1007575. doi: 10.1371/journal.ppat.1007575

Fig 3. Degradation of NHERF1 by 16E6 requires proteasome function.

Fig 3

Keratinocytes retrovirally transduced with either vector or 16E6_WT were seeded at equal confluency. Cells were treated with DMSO, mitomycin C (MMC), or the proteasome inhibitor MG132 at varying concentrations for 8 hours as indicated. MG132 significantly rescued NHERF1 protein levels in a dose dependent manner. MMC treatment was used to induce p53 levels, which were observed as a positive control. Quantification was normalized to vector-transduced cells treated with DMSO. The means of triplicate independent experiments ± standard error are shown. N = 3, *<0.05, **<0.01, ***<0.001, n.s. = no significance by Student’s t-test for samples compared to untreated 16E6 keratinocytes (lane 3).