Skip to main content
. 2019 Apr 19;15(4):e1007575. doi: 10.1371/journal.ppat.1007575

Fig 5. 16E6 mutagenesis screen identified mutants selectively defective in their ability to degrade NHERF1.

Fig 5

(A) Amino acids F69 and K72 are important for degradation of NHERF1 by 16E6. Plasmids encoding untagged 16E6_WT or 16E6 mutants (0.3 ug) were co-transfected with FLAG_E6AP (0.35 ug), HA_NHERF1 (0.4 ug), MYC_p53 (0.25 ug), and HA_GFP (0.08 ug) into C33A cells and HA_NHERF1 levels determined by western blot. Multiple 16E6 proteins were identified that were unable to degrade NHERF1 but were still capable of degrading p53. (B) HA_NHERF1 and (C) p53 protein levels were quantified and normalized to co-transfected HA_GFP as an internal transfection control.