. 2019 Mar 11;100(5):1134–1140. doi: 10.4269/ajtmh.18-0675

Table 2.

Overview of diagnostic indices of the optimal optical density (OD) cutoffs for IgM ELISAs, and patient positivity using the specified cutoff

Criteria		ELISA		Cutoff OD	Sensitivity	Specificity	Accuracy	AUROCC (95% CI)
Criteria		+	−	Cutoff OD	Sensitivity	Specificity	Accuracy	AUROCC (95% CI)
Admission
PCR	+	16	6	1.85	72.7	94.7	91.1	0.81 (0.67, 0.95)
PCR	−	6	107	1.85	72.7	94.7	91.1	0.81 (0.67, 0.95)
Culture	+	4	3	2.16	57.1	88.3	83.0	0.63 (0.31, 0.94)
Culture	−	15	113	2.16	57.1	88.3	83.0	0.63 (0.31, 0.94)
IFA adm ≥ 3,200	+	18	2	2.02	90.0	98.3	97.0	0.94 (0.84, 1.00)
IFA adm ≥ 3,200	−	2	113	2.02	90.0	98.3	97.0	0.94 (0.84, 1.00)
IFA adm ≥ 3,200 or 4-fold rise ≥ 3,200 conv	+	20	5	1.85	80.0	98.2	94.8	0.89 (0.78, 1.00)
IFA adm ≥ 3,200 or 4-fold rise ≥ 3,200 conv	−	2	108	1.85	80.0	98.2	94.8	0.89 (0.78, 1.00)
mSTIC	+	21	12	1.85	63.6	99.0	90.4	0.77 (0.65, 0.90)
mSTIC	−	1	101	1.85	63.6	99.0	90.4	0.77 (0.65, 0.90)
Convalescent
PCR	+	17	5	2.03	77.3	91.2	88.2	0.80 (0.66, 0.94)
PCR	−	10	103	2.03	77.3	91.2	88.2	0.80 (0.66, 0.94)
Culture	+	5	2	2.22	71.4	85.2	84.4	0.67 (0.36, 0.98)
Culture	−	19	109	2.22	71.4	85.2	84.4	0.67 (0.36, 0.98)
IFA adm ≥ 3,200	+	18	2	2.03	90.0	92.2	91.9	0.92 (0.82, 1.00)
IFA adm ≥ 3,200	−	9	106	2.03	90.0	92.2	91.9	0.92 (0.82, 1.00)
IFA adm ≥ 3,200 or 4-fold rise ≥ 3,200 conv	+	22	3	2.03	88.0	95.5	94.1	0.90 (0.80, 1.00)
IFA adm ≥ 3,200 or 4-fold rise ≥ 3,200 conv	−	5	105	2.03	88.0	95.5	94.1	0.90 (0.80, 1.00)
mSTIC	+	23	10	2.03	69.7	96.1	89.6	0.77 (0.64, 0.90)
mSTIC	−	4	98	2.03	69.7	96.1	89.6	0.77 (0.64, 0.90)

AUROCC = area under the receiver operator characteristic curve; IFA = immunofluorescence assay; mSTIC = modified scrub typhus infection diagnostic criteria.