Figure 6.

Fluconazole (Flu) decreases urine output in tolvaptan (Tol)-treated wild-type mice. Wild-type C57bl/6N male mice were treated every 24 hours over a period of 96 hours with Flu (intraperitoneal injections; 80 mg/kg) or 0.9% NaCl. (A) Twenty-four hours before euthanasia, the mice were placed in metabolic cages, and single intraperitoneal injections (20 mg/kg) of Tol were administered to the 0.9% NaCl– and Flu-treated mice (Supplemental Figure 1); 24-hour urine osmolality and output were monitored (n=5 per each group). Statistically significant differences are indicated (mean±SEM). (B and C) Aquaporin-2 (AQP2), RhoA, and phosphorylation of AQP2 and RhoA were detected by Western blotting. The Western blots were quantified as described in Figures 2, 4, and 5. Shown are results from three mice for each condition. *P≤0.05; **P≤0.01; ***P≤0.001.