Figure 3.
Ipragliflozin, but not pioglitazone, ameliorates Kelch-like 3 (KLHL3) phosphorylation in db/db mice. (A) Levels of KLHL3S433-P and total KLHL3 in the kidneys were measured the indicated groups. Blots show biologic replicates. Dot plot graphs show the results of quantitation. (B and C) Immunofluorescence microscopy of (B) NaCl cotransporter (NCC) and (C) KLHL3S433-P in the kidney of indicated animals. Glo, glomerulus. (D and E) Effects of ipragliflozin and pioglitazone on the active phosphorylated form of (D) protein kinase C (PKC) and (E) Akt in db/db mice. Dot plot graphs show the results of densitometric quantitation. Data are expressed as means±SEM; n=5 for db/db and db/db plus ipragliflozin, and n=4 for db/db plus pioglitazone.