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. 2019 Mar 26;30(5):782–794. doi: 10.1681/ASN.2018070703

Figure 7.

Figure 7.

Protein kinase C (PKC) is involved in the dysregulation of Kelch-like 3 (KLHL3) and NaCl cotransporter (NCC) in db/db mice. (A) Effects of bisindolylmaleimide I hydrochloride (BIM), the PKC inhibitor, on high glucose (HG)–induced KLHL3S433-P in human embryotic kidney (HEK) cells. Blots show biologic replicates. Dot plot graphs show the results of densitometric quantitation. (B) Effects of BIM on KLHL3S433-P levels in HEK cells incubated in a low-glucose (LG) medium. Blots show biologic replicates. Dot plot graphs show the results of densitometric quantitation. (C) Effects of BIM on KLHL3S433-P levels in the kidneys of db/db mice. Blots show biologic replicates. Dot plot graphs show the results of densitometric quantitation. (D) Effects of BIM on NCC in the plasma membrane–enriched fraction (PM) of the kidneys in db/db mice. Blots show biologic replicates. Dot plot graphs show the results of densitometric quantitation. (E) Effects of BIM on urinary Na+ levels in the db/db mice. Data are expressed as means±SEM.