Table 1.
Effects of gallic acid and derivatives against toxicity induced by a 24‐h exposure to 25 μM of Aβ25‐35 (Sytox® green assay) in comparison to their inhibitory action on Aβ fibril formation produced by a 24‐h incubation with 15 μM Aβ1‐42 (ThT binding assay)
| Treatment | Sytox® green values (% of control) | Fibrillar forms of Aβ1‐42 (% of control) |
|---|---|---|
| Control | 100 ± 2 | 100 ± 4 |
| Aβ | 190 ± 6 | 686 ± 109 |
| + Gallic acid (5 μM) | 158 ± 4** | 436 ± 46* |
| + Gallic acid (10 μM) | 163 ± 8** | 389 ± 17** |
| + Gallic acid (20 μM) | 134 ± 5** | 246 ± 16** |
| Control | 100 ± 3 | 100 ± 4 |
| Aβ | 190 ± 11 | 637 ± 34 |
| + Tannic acid (1 μM) | 167 ± 10 | 374 ± 4** |
| + Tannic acid (2 μM) | 146 ± 8** | 340 ± 8** |
| + Tannic acid (5 μM) | 115 ± 7** | 288 ± 34** |
| Control | 100 ± 2 | ND |
| Aβ | 131 ± 4 | ND |
| + n‐propyl gallic acid (1 μM) | 126 ± 4 | ND |
| + n‐propyl gallic acid (5 μM) | 117 ± 3** | ND |
| + n‐propyl gallic acid (10 μM) | 108 ± 4** | ND |
| + n‐propyl gallic acid (20 μM) | 93 ± 3** | ND |
| Control | 100 ± 2 | ND |
| Aβ | 124 ± 3 | ND |
| + Pyrogallol (1 μM) | 120 ± 3 | ND |
| + Pyrogallol (5 μM) | 111 ± 3* | ND |
| + Pyrogallol (10 μM) | 103 ± 3** | ND |
| + Pyrogallol (20 μM) | 103 ± 3** | ND |
| Control | 100 ± 1 | 100 ± 3 |
| Aβ | 146 ± 5 | 425 ± 92 |
| + Gallic acid methyl ester (1 μM) | 140 ± 7 | 379 ± 98 |
| + Gallic acid methyl ester (5 μM) | 137 ± 9 | 436 ± 112 |
| + Gallic acid methyl ester (10 μM) | 129 ± 4 | 400 ± 99 |
| + Gallic acid methyl ester (20 μM) | 136 ± 3 | 284 ± 34 |
ND, not determined.
Values represent mean ± SEM of three (MTT) or two (ThT) separate experiments.
*P < 0.05, **P < 0.01 compared with groups treated with Aβ25‐35 alone.