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. 2019 May 1;14(5):e0216218. doi: 10.1371/journal.pone.0216218

Fig 3. Expression of DNMTs in TNFα-treated human endothelial cells.

Fig 3

(A-C) Time course of the effects of TNFα (10 ng/mL) on DNMT1 (A), DNMT3a (B) and DNMT3b (C) mRNA levels in human endothelial cells relative to a triplet of housekeeping genes (HKG); all n = 6 independent cell batches (two-way ANOVA/Bonferroni). (D-E) Effect of RG-108 (RG, 30 μmol/L) on the expression of ACE in human endothelial cells treated with solvent (Sol) or TNFα (10 ng/mL). Time course of ACE mRNA (D); n = 4 independent cell batches (two-way ANOVA/Bonferroni) and ACE protein (E) relative to non-muscle myosin heavy chain (NMM) 24 hours after treatment; n = 3 independent cell batches. **P<0.01, ***P<0.001.