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. 2019 May 1;14(5):e0216218. doi: 10.1371/journal.pone.0216218

Fig 4. Involvement of DNMTs in TNFα-mediated alterations in ACE expression in human endothelial cells.

Fig 4

(A) Consequences of DNMT1 downregulation (si1) on solvent (Sol) and TNFα (10 ng/mL, 24 hours)-induced changes in ACE mRNA relative to a triplet of housekeeping genes (HKG). A scrambled (Scr) oligonucleotide was used as control, n = 4–6 independent cell batches (two-way ANOVA/Bonferroni). (B&C) Consequences of DNMT3a (si3a) and 3b (si3b) downregulation on solvent (Sol) and TNFα (10 ng/mL, 24 hours)-induced changes in ACE mRNA (B) and protein (C) expression. A scrambled (Scr) oligonucleotide was used as control, n = 5–9 independent cell batches (two-way ANOVA/Bonferroni). *P<0.05, **P<0.01, ***P<0.001.