Figure 5.

(A) Expression of PCMT in actin-Pcmt flies. Adult progeny of a cross between the actin 5C-GAL4 driver strain (Bloomington stock 4414) and the X-chromosome UAS-Pcmt responder strain were maintained at 25 (○) or 29°C (●). For the 29°C group, progeny were transferred from 25 to 29°C within several hours after adult eclosion. Activities were normalized to PCMT levels measured in flies of the homozygous parental responder strain maintained at the same temperatures for the same period. (B and C) PCMT overexpression driven by the actin5C promoter extends the lifespan of flies at 29°C. (B) Survival at 25°C. ●, survival of actin-Pcmt flies; ○, survival of the parental X-chromosome UAS-Pcmt responder strain. (C) Survival at 29°C. The meanings of ● and ○ are the same as described in B. ▴, survival of actin5C-GAL4 bearing progeny from an outcross between the driver strain and w¹¹¹⁸/w¹¹¹⁸ flies.