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Photopatterning full-length proteins in hydrogels. The protein of interest is first functionalized with NHS-ortho-nitrobenzyl (o-NB)-CHO and then incorporated into SPAAC-based hydrogels via photomediated oxime ligation. Upon further light exposure, the photoscissile o-NB moieties undergo photocleavage, leading to the removal of linked proteins. Reprinted with permission from ref 419. Copyright 2015 Nature Publishing Group.
