Table 3:

Implementation options for (a) five highest yielding pathways and (b) five shortest pathways. The columns list the pathway yield, length, reaction numbers (using KEGG reaction identification number) in the pathway, the number of sequences with solubility confidence score that were identified using ProSol DB for each reaction step, the maximum solubility confidence score value per reaction step, and bottleneck solubility for this path. Entries in parentheses indicate that multiple enzymes catalyze the reaction step, and in this case the number of sequences and the maximum solubility is reported per enzyme for that reaction step. A “−” indicates that no sequence was identified in both ProSol DB and non-reviewed sequences in UniProtKB.

(a)
Pathway yield	Pathway length	Names of reactions in pathway	# sequences per reaction	Max sol. conf. score per step
0.408	5	3-Hydroxypropionate:CoA ligase (AMP-forming) 3-Hydroxypropionyl-CoA:NADP+ oxidoreductase 3-Oxopropionyl-CoA hydrolase 3-Oxopropanoate:NADP+ oxidoreductase Acetyl-CoA:malonate CoA- transferase	2 350 1 − −	34 100 42 − −
0.407	3	3-Hydroxypropionate:CoA ligase (AMP-forming) 3-Hydroxypropionyl-CoA hydro-lyase Propanoyl-CoA:NADP+ 2-oxidoreductase	2 255, 1 5, 163	34 100, 37 95, 100
0.407	3	3-Hydroxypropionyl-CoA synthetase (ADP-forming) 3-Hydroxypropionyl-CoA hydro-lyase Propanoyl-CoA:NADP+ 2-oxidoreductase	159 255, 1 5,163	100 100, 37 95, 100
0.397	3	3-Hydroxypropionyl-CoA synthetase (ADP-forming) 3-Hydroxypropionyl-CoA hydro-lyase Propanoyl-CoA:NAD+ oxidoreductase	159, 255, 1 1	100 100, 37 99
0.391	5	KEGG R03158 (no name), 3-Hydroxypropionyl-CoA:NADP+ oxidoreductase 3-Oxopropionyl-CoA hydrolase 3-Oxopropanoate:NAD+ oxidoreductase Acetyl-CoA:malonate CoA-transferase	14 350 1 − −	100 100 42 − −
(b)
Pathway yield	Pathway length	Names of reactions in pathway	# sequences per reaction	Max sol. conf. score per step
0.373	2	3-Hydroxypropanoate:NAD+ oxidoreductase, L-Alanine:3-oxopropanoate aminotransferase	− 2	− 90
0.373	2	3-Hydroxypropanoate:NAD+ oxidoreductase, L-Alanine:3-oxopropanoate aminotransferase	− 2	− 90
0.373	2	3-Hydroxypropanoate:NAD+ oxidoreductase, beta-Alanine:2-oxoglutarate aminotransferase	− 120	− 99
0.367	2	3-Hydroxypropanoate:NAD+ oxidoreductase, 3-Oxopropanoate:NADP+ oxidoreductase (CoA-malonylating)	− 2	− 74
0.365	2	3-Hydroxypropionate:NADP+ oxidoreductase, L-Alanine:3-oxopropanoate aminotransferase	386 2	100 90
0.365	2	3-Hydroxypropionate:NADP+ oxidoreductase, beta-Alanine:2-oxoglutarate aminotransferase	386 20	100 99