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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Breast Cancer Res Treat. 2019 Feb 2;175(1):39–50. doi: 10.1007/s10549-019-05146-7

Figure 1. Methionine restriction inhibits CSCs by a SAM-dependent mechanism.

Figure 1.

a, Mammosphere assay in TNBC cells. MDA-MB-231, GILM2 and BT20 TNBC cells were cultured in ultra-low attachment plates in normal (control) or methionine-free mammosphere growth media (0% Met) with or without SAM (100 μM). Top panel, representative images showing mammospheres at day 7. Bottom panel, mammospheres were scored in ten fields from each well (mean ± SEM, n =3) *, P < 0.05, ***, P < 0.001 versus mammospheres cultured in control media without SAM. b, Flow cytometry analysis of CD44 and CD24 expression in primary mammospheres from TNBC cells grown for 6 days in control or methionine-free media with or without SAM (100 μM).