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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Breast Cancer Res Treat. 2019 Feb 6;175(1):77–90. doi: 10.1007/s10549-018-05126-3

Figure 3. ID2 promotes DCIS formation in vivo.

Figure 3.

(a) MCF10AT-ID2 and MCF10AT-pCDH (control) cells were implanted in the same mouse into the left and right side of the mammary fat pad, respectively (n = 10, left panel). (b) Tumor growth was monitored with the IVIS imaging system for up to 3 weeks. Representative IVIS images on day 1 and day 21 are shown in the right panels. The growth kinetics of tumor are shown in (c). (d) The tumors generated by MCF10AT-ID2 cells in nude mice were sectioned and H&E stained (left panel). Tumor formed by MCF10AT showed strong ID2 expression. Scale bar, 100μm. (e) DCIS-shCTRL and DCIS-shID2 cells were implanted into the mammary fat pad and monitored with the IVIS imaging system for 3 weeks. Representative images of tumor signal at day 21. (f) Growth kinetic of tumors. (g) The tumors generated by DCIS-shCTRL and DCIS-shID2 cells in nude mice were sectioned and H&E stained. Scale bar, 100μm.