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. 2001 Dec 4;98(26):14849–14852. doi: 10.1073/pnas.261517398

Figure 1.

Figure 1

The gal promoter region of E. coli showing the P1 and P3 promoters in the plasmid pSA852 (D. Lewis, unpublished data). pSA852 contains a 288-bp segment of the gal control region (−197 to +91) followed by a transcription terminator (21). The CRP binding site is in bold. The transcription start site of P1 is denoted as +1. The −10 region, the P1 promoter, is in bold and boxed, and that of P3 is boxed. The adenine residues with the asterisks were replaced individually by 2-AP in the 106-base-long rightward primer. DNA templates (385 bp) for in vitro transcription containing 2-AP were generated by PCR using the rightward and leftward primers (shown by arrows) from pSA852. The 106-base-long primer also contained −20T to G and −19T to G changes to inactivate the P2 promoter. T, transcription termination signal.