A804598 treatment reversed significant increases in the protein levels of pro-IL1β, the precursor of IL-1β, and p65 NF-kB1, a component of NF-kB1 complex. Upper panels - Western blots of representative bands for the proteins. Lower panels – densitometry analyses of the pro-IL-1β (a) and NF-kB1 (b) levels normalized to their corresponding actin levels. Data are presented as fold differences from the Control values, which were averaged and set as “1”. Data are reported as mean ± SEM. * P < 0.05, ** P < 0.01 compared to Control, # P < 0.05, # P < 0.01 compared to Hybrid, (n = 5/Control, n = 4 for Hybrid and Hybrid + A804598)