Figure 3. Enrichment of mature peripheral iNKT cells by depletion of CD24⁺ splenocytes. (A) Identification of CD24⁺ cells by surface TCRβ and B220 staining. Dot plots are representative of 2 independent experiments. (B) Mature iNKT cell enrichment from C57BL/6 spleen cells by magnetic-activated depletion of CD24⁺ cells. Dot plots (top) are representative and graph (bottom) shows summary of 3 independent experiments. (C) Surface CD69 and CD5 staining on total and CD24⁺ cell depleted BALB/c spleen iNKT cells upon PMA + ionomycin stimulation. Results are representative of 2 independent experiments.

^**p<0.01 were considered statistically significant.