Figure 2. Activation of MAPK and NF-κB by ESAT6 are involved in TLR4-dependent manner in BMDCs. WT and TLR4-deficient BMDCs were treated with ESAT6 (50 ng/ml) for the indicated time points, and cellular proteins were extracted. The phosphorylation of ERK, JNK, p38, and p65 and IκB-α degradation were analyzed through Western blotting (A-F). Antibody against β-actin was used to confirm the loading doses.
