Figure 4. TLR4 in BMDCs increases the expression of marker of DCs maturation and activated by ESAT6 play an important role in production of IFN-γ and IL-17 in CD4⁺ T cell. WT, TLR2-, and TLR4-deficient BMDCs were treated with ESAT6 (50 ng/ml) for 24 h. The expression of CD80, CD86, and MHC class II was analyzed by flow cytometry (A). WT, TLR4-, and TLR2-deficient cells were pretreated with ESAT6 (50 ng/ml) with OVA_323-339 (OT-II peptide) for 24 h, then co-cultured with naïve CD4⁺ T cells for five days. The concentration of IFN-γ and IL-17 in supernatant was measured by ELISA (B and C). Data are presented as mean ± SD.

^**p<0.01; ^***p<0.001 vs. control cells.