Figure 3. IM156 dose-dependently suppresses the differentiation of central memory T cells and effector memory T cells.

LCMV-infected mice were treated with various concentrations of IM156 from days −1 to 29 post-infection. (A) Representative data showing the frequency of GP33 tetramer-positive cells among CD8⁺ or CD4⁺ T cells in the spleen at day 30 post-infection. (B) Frequency (top) and absolute number (bottom) of GP33⁺CD8⁺ T cells as in A. (C) Co-expression of either KLRG1 (left) or CD62L (right) and CD127 on tetramer-positive CD8⁺ T cells obtained from the spleen at day 30 post-infection. (D) Frequency (left) and absolute number (right) of MPECs (CD127^hi KLRG1^lo) and SLECs (CD127^loKLRG1^hi) as in C. (E) Frequency (left) and absolute number (right) of T_CM (CD127⁺CD62L⁺), T_EM (CD127⁺CD62L⁻), and T_EFF (CD127⁻CD62L⁻) as in C. Data are representative of 3 independent experiments n=4–5 mice per group. Results are the mean ± SEM and statistical significance was determined by 2-tailed unpaired Student's t-test.

^*p<0.05; ^**p<0.01; ^***p<0.001.