Figure 4. IM156 inhibits multi-functional CD8⁺ and CD4⁺ T cells.

LCMV-infected mice were treated with various concentrations of IM156 from days −1 to 29 post-infection. (A) Representative flow cytometric analysis of production of IFN-γ, TNF-α, and IL-2 in CD8⁺ T cells obtained from the spleen at day 30 post-infection after ex vivo re-stimulation with GP33 peptide. (B) Frequency of TNF-α-producing cells (top left) and IL-2-producing cells (top right) among IFN-γ⁺CD8⁺ T cells. MFI of TNF-α (bottom left) and IL-2 (bottom right) among IFN-γ⁺CD8⁺ T cells as in A. (C) Representative flow cytometric analysis of production of IFN-γ, TNF-α, and IL-2 in CD4⁺ T cells obtained from the spleen at day 30 post-infection after ex vivo re-stimulation with GP66 peptide. (D) Frequency of TNF-α-producing cells (top left) and IL-2-producing cells (top right) among IFN-γ⁺CD4⁺ T cells. MFI of TNF-α (bottom left) and IL-2 (bottom right) among IFN-γ⁺CD4⁺ T cells as in C. Data are representative of 3 independent experiments n=4–5 mice per group. Results are the mean ± SEM and statistical significance was determined by 2-tailed unpaired Student's t-test.

^*p<0.05; ^**p<0.01; ^***p<0.001.