Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 1;9:6776. doi: 10.1038/s41598-019-43255-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PFBC XPR1/SLC53A1 variants do not alter virus entry. (a) Cell surface detection of XPR1/SLC53A1 (upper panel) and GLUT1/SLC2A1 (lower panel) in CHO hamster cell populations stably expressing the control parental expression vector lacking XPR1/SLC53A1 (LNCX), or encoding either WT XPR1/SLC53A1, or variants p.R459C, p.N619D or p.I629S, or the p.(L612_T696del) deletion mutant. Cells were assayed for XPR1/SLC53A1 and GLUT1/SLC2A1 surface expression with their corresponding XRBD and H2RBD ligands, respectively. Numbers indicate the specific delta mean fluorescence intensity of a representative experiment (n = 3), as compared to non-specific staining with the secondary IgG (grey histograms). (b) Representative immunoblot of HA-tagged XPR1/SLC53A1-containing cell lysates from (a) with an anti-HA antibody (upper panel), or with an anti β-actin antibody used as loading control (lower panel). (c) Analysis of viral infection in CHO cells stably expressing either of WT or XPR1/SLC53A1 variants. Cells were assayed for infection with retroviral vectors pseudotyped with X-MLV Env or (d) VSV-G. Two days after infection, cells were analyzed by flow cytometry. Graphics are average values from 3 different wells in one representative experiment (n = 3). (e) Infectivity of viral supernatants on control HEK293T susceptible cells.