Fig. 2.

CXCL4 forms complexes with different DNA types and protects DNA from nucleases. a HuDNA or bacDNA (10 μg ml⁻¹) were stained with PicoGreen and mixed with different concentrations of the indicated molecules. Fluorescence emission was measured by a fluorimeter. Data are expressed as percent of fluorescence with respect to the fluorescence of DNA alone (100%). Horizontal bars represent the mean, vertical bars are s.e.m. Results from eight independent experiments performed with either huDNA or bacDNA (four each); *P-values < 0.05 by Student’s t test for paired samples (two-tailed) are calculated with respect to the fluorescence of DNA alone; b 10 μM of the indicated proteins were premixed with 10 μg of fluorochrome-conjugated huDNA. Formation of complexes was visualized by confocal microscopy; no binding resulted in a dark panel. One representative experiment out of four. c HuDNA or bacDNA (10 μg ml⁻¹) were mixed with different doses of the indicated proteins for 20 min in the presence/absence of DNase I (see Methods). Fluorescence was analyzed by PicoGreen assay and percent of DNA protected from degradation calculated with respect to DNA degradation (decrease of picoGreen fluorescence) obtained in the absence of any molecule (DNA alone). Horizontal bars are the mean, vertical bars are s.e.m. Results from six independent experiments performed with huDNA or bacDNA (three each). *P-values < 0.05 by Student’s t test for paired samples (two-tailed) calculated in comparison with degradation of DNA alone