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. 2019 May 1;9:6751. doi: 10.1038/s41598-019-43230-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Romidepsin treatment does not affect cell proliferation at 3 hpa. (A) Embryo tail images showing EdU and DAPI staining at 3 hpa for control (DMSO) and treatment groups (Romi 0–1 mpa, and Rmoi 0–3 hpa). Scale bar = 200 μ. (B) Representative image for the 200 μ area of the tail tip used for cell counts and calculations. (C) EdU quantification for embryos treated with DMSO (N = 6) and Romidepsin for 0–1mpa (N = 6) and 0–3 hpa (N = 6). Error bars are standard deviations of the mean.