Fig. 3.

Glycan expression technology (GET). To express foreign sugar structures in E. coli, first the genes responsible for synthesising the glycan must be cloned. The example here is CPS I coding locus of B. pseudomallei K96243. Expression of these genes leads to synthesis and export of the foreign sugar attached to lipid A, in the absence of the native O-antigen from E. coli. a–d show Immunofluorescence microscopy of E. coli cells coated with the new sugar structures. Cells were probed with group or type-specific anti-glycan antibodies and Alexa Fluor 488 conjugated secondary antibody. A, unpublished; B, reproduced from Cuccui et al.⁷⁰; c, d reproduced from Kay et al.⁶⁶