Fig. 3. YD can regulate the degradation of AXL and effectively inhibit the growth of both EGFR-TKI sensitive and resistant cells.

Chemical structure of yuanhuadine (YD) (a). Indicated cell lines were treated with YD for 72 h andviability was measured. (b). The protein expression of AXL (140 kda) was detected after co-treatmentof CHX and YD by Western blotting analysis over the indicated time points to test the effects of YD onthe protein degradation. The expressions of AXL and β-actin were quantified by densitometry usingImageJ. AXL expressions were normalized to β-actin and further compared with 0 h expression (c). Cells were treated with indicated concentrations of YD for 24 h (d). Data are presented as the mean foldchanges ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.005 by t-test