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. 2019 May 1;9:6749. doi: 10.1038/s41598-019-43233-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ERK 1/2 (a), phosphoERK 1/2 (b), MEK 1 (c) and MEK 2 (d) protein level and representative immunoblot of ERK 1/2, phosphoERK 1/2, MEK 1 and MEK 2 protein analysis (f) in colon section obtained from control, TNBS and treated groups. Representative images of immunohistochemical staining of GPER and phosphoERK 1/2 (e) in colon cross-section obtained from control, TNBS and treated groups – type of treatment over each images are shown. Data are presented as means ± SEM; *P < 0.05, ***P < 0.001 TNBS vs. control; ^#P < 0.05, ^##P < 0.01 treated groups vs. TNBS; ^&&&P < 0.001 G-15 treated group vs. G-1 treated group. 10–15 mice per group. Ten independent cross-section per group were examined. Scale bars, 50 µm. Representative blots were cropped from the same gels.