Fig. 1. PRMT1 is predominantly expressed in ESCC and positively associated with OV6 expression.

a, b ONCOMINE (a) and CCLE (b) data showed that PRMT1 was highly expressed in ESCC patients and cell lines. c Western blot and qRT-PCR data showed the elevated PRMT1 expression in ECA109 and TE1 cell lines compared with HEEC cells. d PRMT1 expression in ESCC cancer tissue compared with adjacent animal tissue (scare bar = 20 µm). e Overall survival curve of patients with negative PRMT1 expression (blue line) and patients with positive PRMT1 expression (red line), P = 0.0157. f 95 ESCC patient samples were used for immunohistochemistry analysis of the expression of PRMT1 and OV6. Representative staining intensity of PRMT1 and OV6 expression represents different expression level (×200, scale bar = 100 µm; ×400, scale bar = 20 µm). g The overall survival rates of 95 patients with ESCC were compared among different groups, P = 0.0006. h, i The percentage of patients with high OV6 staining is higher in the PRMT1-high group (upper panel). Spearman correlation analysis shows a significant correlation of the expression levels of PRMT1 and OV6 in ESCC (lower panel), P = 0.007 r = 0.276