Skip to main content
. 2019 May 1;9:6759. doi: 10.1038/s41598-019-42894-4

Figure 7.

Figure 7

TXA2-R trafficking in young versus old RIAS SMC by reversible biotinylation. IAS SMC membranous receptors were labeled using biotin and incubated with their respective agonists at 37 °C for varying amounts of time, after which the cells were lysed with RIPA. Biotinylated proteins affinity-purified from cell lysate using streptavidin-agarose monitored via WB (A,B) and confocal microscopy (C) show that aging induces faster internalization at early phase of receptor trafficking (represented after 1 h incubation) and slow recycling of cell-surface receptors. The receptor recycling rate was significantly increased (representing rescue from internalization) following pre-incubation of the aging RIAS SMC with chloroquine (lysomotoropic agent) and MG132 (proteasomal inhibitor) (both 10 μM), as determined by normalizing the cell surface receptor density at 0 h and 5 h time points (D,E) (*P < 0.05; n = 4).