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. 2019 May 1;9:6777. doi: 10.1038/s41598-019-43166-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fusion model of all the LPS aliquots after variable selection. The left panel (a) shows the LPS response samples in blue and the control samples in red. If the prediction score value is above the threshold, the samples were classified as a LPS responder. The three panels on the right (b–d) show the weights in the model of, respectively, aliquots 1, 2 and 3. Positive weights are coloured blue and belong to cells more represented in the LPS response samples, while negative weights are coloured red and belong to cells more abundant in the control samples. The arrows show the loadings and thus the marker expression. CM indicates mature neutrophils more abundant in the controls, while LM and LI respectively indicate mature and immature neutrophils more abundant in the LPS responders.